In this letter Crick refers to genetic experiments undertaken with Leslie Barnett, Sydney Brenner, and Richard Watts-Tobin on acridine mutants, which indicated that the genetic code was a triplet code. Compelling evidence that poly-U (a synthetic form of RNA consisting of bases of a single type, uracil) coded for the amino acid phenylalanine was first presented by the American biochemist Marshall Nirenberg in the summer of 1961.
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