Acceleration of Reactivation of Reduced Bovine Pancreatic Ribonuclease by a Microsomal System from Rat Liver
- Contributor(s):
- Goldberger, Robert E., 1937- Epstein, Charles Anfinsen, Christian B. (Christian Boehmer), 1916-1995 Journal of Biological Chemistry
- Publication:
- American Society for Biochemistry and Molecular Biology, February 1963
- Language(s):
- English
- Format:
- Text
- Subject(s):
- Ribonucleases Microsomes Oxidation-Reduction Enzymes Research Design
- Genre(s):
- Archival Materials Articles
- Abstract:
- Prior studies to this article by Anfinsen, Epstein, and Goldberger established that the time required for synthesizing a chain of protein such as ribonuclease in the tissues of higher organisms would be approximately two minutes. Here, the three researchers noted that an enzyme system in the endoplasmic reticulum of cells that catalyzed the disulfide interchange reaction and which, when added to solutions of reduced ribonuclease, catalyzed the rapid formation of the native disulfide pairing in a period less than two minutes. Subsequent studies would find that such motile proteins as staphylococcal nuclease or myoglobin could undergo virtually complete renaturation in only a few seconds or less.
- Copyright:
- This item may be under copyright protection; contact the copyright owner for permission before re-use.
- Extent:
- 8 pages
- NLM Unique ID:
- 101584571X144 (See in Profiles in Science)
- Profiles in Science ID:
- KKBBJX
- Permanent Link:
- http://resource.nlm.nih.gov/101584571X144
- Archival Collection:
- The Christian B. Anfinsen Papers (Profiles in Science)